DNA

Part:BBa_K1195008:Design

Designed by: Clarice Harrison   Group: iGEM13_BYU_Provo   (2013-08-28)


promoter FtSQAZ + GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1182
    Illegal EcoRI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 462
    Illegal SpeI site found at 1183
    Illegal PstI site found at 1197
    Illegal NotI site found at 1190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1183
    Illegal EcoRI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 462
    Illegal SpeI site found at 1183
    Illegal PstI site found at 1197
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

E. coli

References

Yao, Yong, et al. "Structure of the< i> Escherichia coli</i> Quorum Sensing Protein SdiA: Activation of the Folding Switch by Acyl Homoserine Lactones." Journal of molecular biology 355.2 (2006): 262-273.

Sitnikov, Dmitry M., Jeffrey B. Schineller, and Thomas O. Baldwin. "Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction." Proceedings of the National Academy of Sciences 93.1 (1996): 336-341.

Wei, Yan, et al. "Global impact of sdiA amplification revealed by comprehensive gene expression profiling of Escherichia coli." Journal of bacteriology 183.7 (2001): 2265-2272.